Synaptic Partner Assignment Using Attentional Voxel Association Networks

Connectomics aims to recover a complete set of synaptic connections within a dataset imaged by electron microscopy. Most systems for locating synapses use voxelwise classifier models, and train these classifiers to reproduce binary masks of synaptic clefts. However, only recent work has included a way to identify the synaptic partners that communicate at synaptic cleft segments. Here, we present a novel method for associating synaptic cleft segments with their synaptic partners using a convolutional network trained to associate the mask of a cleft with the voxels of its synaptic partners. The network takes the local image context and a mask of a single cleft segment as input. It is trained to produce two volumes of output: one which labels the voxels of the presynaptic partner within the input image, and another similar volume for the postsynaptic partner. The cleft mask acts as an attentional gating signal for the network, in that two clefts with the same local image context often have different partners. We find that an implementation of this approach performs well on a dataset of mouse somatosensory cortex, and evaluate it as part of a combined system to predict both clefts and connections.

Deep Learning Improves Template Matching by Normalized Cross Correlation

Template matching by normalized cross correlation (NCC) is widely used for finding image correspondences. We improve the robustness of this algorithm by preprocessing images with "siamese" convolutional networks trained to maximize the contrast between NCC values of true and false matches. The improvement is quantified using patches of brain images from serial section electron microscopy. Relative to a parameter-tuned bandpass filter, siamese convolutional networks significantly reduce false matches. Furthermore, all false matches can be eliminated by removing a tiny fraction of all matches based on NCC values. The improved accuracy of our method could be essential for connectomics, because emerging petascale datasets may require billions of template matches to assemble 2D images of serial sections into a 3D image stack. Our method is also expected to generalize to many other computer vision applications that use NCC template matching to find image correspondences.