Abstract:Deep Learning models have been successfully utilized to extract clinically actionable insights from routinely available histology data. Generally, these models require annotations performed by clinicians, which are scarce and costly to generate. The emergence of self-supervised learning (SSL) methods remove this barrier, allowing for large-scale analyses on non-annotated data. However, recent SSL approaches apply increasingly expansive model architectures and larger datasets, causing the rapid escalation of data volumes, hardware prerequisites, and overall expenses, limiting access to these resources to few institutions. Therefore, we investigated the complexity of contrastive SSL in computational pathology in relation to classification performance with the utilization of consumer-grade hardware. Specifically, we analyzed the effects of adaptations in data volume, architecture, and algorithms on downstream classification tasks, emphasizing their impact on computational resources. We trained breast cancer foundation models on a large public patient cohort and validated them on various downstream classification tasks in a weakly supervised manner on two external public patient cohorts. Our experiments demonstrate that we can improve downstream classification performance whilst reducing SSL training duration by 90%. In summary, we propose a set of adaptations which enable the utilization of SSL in computational pathology in non-resource abundant environments.
Abstract:Deep Learning (DL) can predict biomarkers directly from digitized cancer histology in a weakly-supervised setting. Recently, the prediction of continuous biomarkers through regression-based DL has seen an increasing interest. Nonetheless, clinical decision making often requires a categorical outcome. Consequently, we developed a weakly-supervised joint multi-task Transformer architecture which has been trained and evaluated on four public patient cohorts for the prediction of two key predictive biomarkers, microsatellite instability (MSI) and homologous recombination deficiency (HRD), trained with auxiliary regression tasks related to the tumor microenvironment. Moreover, we perform a comprehensive benchmark of 16 approaches of task balancing for weakly-supervised joint multi-task learning in computational pathology. Using our novel approach, we improve over the state-of-the-art area under the receiver operating characteristic by +7.7% and +4.1%, as well as yielding better clustering of latent embeddings by +8% and +5% for the prediction of MSI and HRD in external cohorts, respectively.
Abstract:Hematoxylin- and eosin (H&E) stained whole-slide images (WSIs) are the foundation of diagnosis of cancer. In recent years, development of deep learning-based methods in computational pathology enabled the prediction of biomarkers directly from WSIs. However, accurately linking tissue phenotype to biomarkers at scale remains a crucial challenge for democratizing complex biomarkers in precision oncology. This protocol describes a practical workflow for solid tumor associative modeling in pathology (STAMP), enabling prediction of biomarkers directly from WSIs using deep learning. The STAMP workflow is biomarker agnostic and allows for genetic- and clinicopathologic tabular data to be included as an additional input, together with histopathology images. The protocol consists of five main stages which have been successfully applied to various research problems: formal problem definition, data preprocessing, modeling, evaluation and clinical translation. The STAMP workflow differentiates itself through its focus on serving as a collaborative framework that can be used by clinicians and engineers alike for setting up research projects in the field of computational pathology. As an example task, we applied STAMP to the prediction of microsatellite instability (MSI) status in colorectal cancer, showing accurate performance for the identification of MSI-high tumors. Moreover, we provide an open-source codebase which has been deployed at several hospitals across the globe to set up computational pathology workflows. The STAMP workflow requires one workday of hands-on computational execution and basic command line knowledge.