COVID-19: post infection implications in different age groups, mechanism, diagnosis, effective prevention, treatment, and recommendations

SARS-CoV-2, the highly contagious pathogen responsible for the COVID-19 pandemic, has persistent effects that begin four weeks after initial infection and last for an undetermined duration. These chronic effects are more harmful than acute ones. This review explores the long-term impact of the virus on various human organs, including the pulmonary, cardiovascular, neurological, reproductive, gastrointestinal, musculoskeletal, endocrine, and lymphoid systems, particularly in older adults. Regarding diagnosis, RT-PCR is the gold standard for detecting COVID-19, though it requires specialized equipment, skilled personnel, and considerable time to produce results. To address these limitations, artificial intelligence in imaging and microfluidics technologies offers promising alternatives for diagnosing COVID-19 efficiently. Pharmacological and non-pharmacological strategies are effective in mitigating the persistent impacts of COVID-19. These strategies enhance immunity in post-COVID-19 patients by reducing cytokine release syndrome, improving T cell response, and increasing the circulation of activated natural killer and CD8 T cells in blood and tissues. This, in turn, alleviates symptoms such as fever, nausea, fatigue, muscle weakness, and pain. Vaccines, including inactivated viral, live attenuated viral, protein subunit, viral vectored, mRNA, DNA, and nanoparticle vaccines, significantly reduce the adverse long-term effects of the virus. However, no vaccine has been reported to provide lifetime protection against COVID-19. Consequently, protective measures such as physical distancing, mask usage, and hand hygiene remain essential strategies. This review offers a comprehensive understanding of the persistent effects of COVID-19 on individuals of varying ages, along with insights into diagnosis, treatment, vaccination, and future preventative measures against the spread of SARS-CoV-2.

Benchmarking the Cell Image Segmentation Models Robustness under the Microscope Optical Aberrations

Cell segmentation is essential in biomedical research for analyzing cellular morphology and behavior. Deep learning methods, particularly convolutional neural networks (CNNs), have revolutionized cell segmentation by extracting intricate features from images. However, the robustness of these methods under microscope optical aberrations remains a critical challenge. This study comprehensively evaluates the performance of cell instance segmentation models under simulated aberration conditions using the DynamicNuclearNet (DNN) and LIVECell datasets. Aberrations, including Astigmatism, Coma, Spherical, and Trefoil, were simulated using Zernike polynomial equations. Various segmentation models, such as Mask R-CNN with different network heads (FPN, C3) and backbones (ResNet, VGG19, SwinS), were trained and tested under aberrated conditions. Results indicate that FPN combined with SwinS demonstrates superior robustness in handling simple cell images affected by minor aberrations. Conversely, Cellpose2.0 proves effective for complex cell images under similar conditions. Our findings provide insights into selecting appropriate segmentation models based on cell morphology and aberration severity, enhancing the reliability of cell segmentation in biomedical applications. Further research is warranted to validate these methods with diverse aberration types and emerging segmentation models. Overall, this research aims to guide researchers in effectively utilizing cell segmentation models in the presence of minor optical aberrations.

Pneumonia App: a mobile application for efficient pediatric pneumonia diagnosis using explainable convolutional neural networks (CNN)

Mycoplasma pneumoniae pneumonia (MPP) poses significant diagnostic challenges in pediatric healthcare, especially in regions like China where it's prevalent. We introduce PneumoniaAPP, a mobile application leveraging deep learning techniques for rapid MPP detection. Our approach capitalizes on convolutional neural networks (CNNs) trained on a comprehensive dataset comprising 3345 chest X-ray (CXR) images, which includes 833 CXR images revealing MPP and additionally augmented with samples from a public dataset. The CNN model achieved an accuracy of 88.20% and an AUROC of 0.9218 across all classes, with a specific accuracy of 97.64% for the mycoplasma class, as demonstrated on the testing dataset. Furthermore, we integrated explainability techniques into PneumoniaAPP to aid respiratory physicians in lung opacity localization. Our contribution extends beyond existing research by targeting pediatric MPP, emphasizing the age group of 0-12 years, and prioritizing deployment on mobile devices. This work signifies a significant advancement in pediatric pneumonia diagnosis, offering a reliable and accessible tool to alleviate diagnostic burdens in healthcare settings.

A self-organizing multi-agent system for distributed voltage regulation

This paper presents a distributed voltage regulation method based on multi-agent system control and network self-organization for a large distribution network. The network autonomously organizes itself into small subnetworks through the epsilon decomposition of the sensitivity matrix, and agents group themselves into these subnetworks with the communication links being autonomously determined. Each subnetwork controls its voltage by locating the closest local distributed generation and optimizing their outputs. This simplifies and reduces the size of the optimization problem and the interaction requirements. This approach also facilitates adaptive grouping of the network by self-reorganizing to maintain a stable state in response to time-varying network requirements and changes. The effectiveness of the proposed approach is validated through simulations on a model of a real heavily-meshed secondary distribution network. Simulation results and comparisons with other methods demonstrate the ability of the subnetworks to autonomously and independently regulate the voltage and to adapt to unpredictable network conditions over time, thereby enabling autonomous and flexible distribution networks.