Machine Learning-Based Prediction of Key Genes Correlated to the Subretinal Lesion Severity in a Mouse Model of Age-Related Macular Degeneration

Age-related macular degeneration (AMD) is a major cause of blindness in older adults, severely affecting vision and quality of life. Despite advances in understanding AMD, the molecular factors driving the severity of subretinal scarring (fibrosis) remain elusive, hampering the development of effective therapies. This study introduces a machine learning-based framework to predict key genes that are strongly correlated with lesion severity and to identify potential therapeutic targets to prevent subretinal fibrosis in AMD. Using an original RNA sequencing (RNA-seq) dataset from the diseased retinas of JR5558 mice, we developed a novel and specific feature engineering technique, including pathway-based dimensionality reduction and gene-based feature expansion, to enhance prediction accuracy. Two iterative experiments were conducted by leveraging Ridge and ElasticNet regression models to assess biological relevance and gene impact. The results highlight the biological significance of several key genes and demonstrate the framework's effectiveness in identifying novel therapeutic targets. The key findings provide valuable insights for advancing drug discovery efforts and improving treatment strategies for AMD, with the potential to enhance patient outcomes by targeting the underlying genetic mechanisms of subretinal lesion development.

Artificial Intelligence Enhanced Rapid and Efficient Diagnosis of Mycoplasma Pneumoniae Pneumonia in Children Patients

Artificial intelligence methods have been increasingly turning into a potentially powerful tool in the diagnosis and management of diseases. In this study, we utilized logistic regression (LR), decision tree (DT), gradient boosted decision tree (GBDT), support vector machine (SVM), and multilayer perceptron (MLP) as machine learning models to rapidly diagnose the mycoplasma pneumoniae pneumonia (MPP) in children patients. The classification task was carried out after applying the preprocessing procedure to the MPP dataset. The most efficient results are obtained by GBDT. It provides the best performance with an accuracy of 93.7%. In contrast to standard raw feature weighting, the feature importance takes the underlying correlation structure of the features into account. The most crucial feature of GBDT is the "pulmonary infiltrates range" with a score of 0.5925, followed by "cough" (0.0953) and "pleural effusion" (0.0492). We publicly share our full implementation with the dataset and trained models at https://github.com/zhenguonie/2021_AI4MPP.