To promote the development of Vision-Language Pre-training (VLP) and multimodal Large Language Model (LLM) in the Chinese community, we firstly release the largest public Chinese high-quality video-language dataset named Youku-mPLUG, which is collected from Youku, a well-known Chinese video-sharing website, with strict criteria of safety, diversity, and quality. Youku-mPLUG contains 10 million Chinese video-text pairs filtered from 400 million raw videos across a wide range of 45 diverse categories for large-scale pre-training. In addition, to facilitate a comprehensive evaluation of video-language models, we carefully build the largest human-annotated Chinese benchmarks covering three popular video-language tasks of cross-modal retrieval, video captioning, and video category classification. Youku-mPLUG can enable researchers to conduct more in-depth multimodal research and develop better applications in the future. Furthermore, we release popular video-language pre-training models, ALPRO and mPLUG-2, and our proposed modularized decoder-only model mPLUG-video pre-trained on Youku-mPLUG. Experiments show that models pre-trained on Youku-mPLUG gain up to 23.1% improvement in video category classification. Besides, mPLUG-video achieves a new state-of-the-art result on these benchmarks with 80.5% top-1 accuracy in video category classification and 68.9 CIDEr score in video captioning, respectively. Finally, we scale up mPLUG-video based on the frozen Bloomz with only 1.7% trainable parameters as Chinese multimodal LLM, and demonstrate impressive instruction and video understanding ability. The zero-shot instruction understanding experiment indicates that pretraining with Youku-mPLUG can enhance the ability to comprehend overall and detailed visual semantics, recognize scene text, and leverage open-domain knowledge.