Knowledge distillation (KD) is a widely adopted and effective method for compressing models in object detection tasks. Particularly, feature-based distillation methods have shown remarkable performance. Existing approaches often ignore the uncertainty in the teacher model's knowledge, which stems from data noise and imperfect training. This limits the student model's ability to learn latent knowledge, as it may overly rely on the teacher's imperfect guidance. In this paper, we propose a novel feature-based distillation paradigm with knowledge uncertainty for object detection, termed "Uncertainty Estimation-Discriminative Knowledge Extraction-Knowledge Transfer (UET)", which can seamlessly integrate with existing distillation methods. By leveraging the Monte Carlo dropout technique, we introduce knowledge uncertainty into the training process of the student model, facilitating deeper exploration of latent knowledge. Our method performs effectively during the KD process without requiring intricate structures or extensive computational resources. Extensive experiments validate the effectiveness of our proposed approach across various distillation strategies, detectors, and backbone architectures. Specifically, following our proposed paradigm, the existing FGD method achieves state-of-the-art (SoTA) performance, with ResNet50-based GFL achieving 44.1% mAP on the COCO dataset, surpassing the baselines by 3.9%.