As the application of Large Language Models (LLMs) expands, the demand for reliable evaluations increases. Existing LLM evaluation benchmarks primarily rely on static datasets, making it challenging to assess model performance in dynamic interactions with users. Moreover, these benchmarks often depend on specific background knowledge, complicating the measurement of a model's logical reasoning capabilities. Other dynamic evaluation methods based on strong models or manual efforts may introduce biases and incur high costs and time demands, hindering large-scale application. To address these issues, we propose TurtleBench. TurtleBench collects real user guesses from our online Turtle Soup Puzzle platform that we developed. This approach allows for the relatively dynamic generation of evaluation datasets, mitigating the risk of model cheating while aligning assessments more closely with genuine user needs for reasoning capabilities, thus enhancing the reliability of evaluations. TurtleBench includes 1,532 user guesses along with the correctness of guesses after annotation. Using this dataset, we thoroughly evaluated nine of the most advanced LLMs available today. Notably, the OpenAI o1 series models did not achieve leading results in these evaluations. We propose several hypotheses for further research, such as "the latent reasoning of o1 utilizes trivial Chain-of-Thought (CoT) techniques" and "increasing CoT length not only provides reasoning benefits but also incurs noise costs."