The EPC GEN 2 communication protocol for Ultra-high frequency Radio Frequency Identification (RFID) has offered a promising avenue for advancing the intelligence of transportation infrastructure. With the capability of linking vehicles to RFID readers to crowdsource information from RFID tags on road infrastructures, the RF-enhanced road infrastructure (REI) can potentially transform data acquisition for urban transportation. Despite its potential, the broader adoption of RFID technologies in building intelligent roads has been limited by a deficiency in understanding how the GEN 2 protocol impacts system performance under different transportation settings. This paper fills this knowledge gap by presenting the system architecture and detailing the design challenges associated with REI. Comprehensive real-world experiments are conducted to assess REI's effectiveness across various urban contexts. The results yield crucial insights into the optimal design of on-vehicle RFID readers and on-road RFID tags, considering the constraints imposed by vehicle dynamics, road geometries, and tag placements. With the optimized designs of encoding schemes for reader-tag communication and on-vehicle antennas, REI is able to fulfill the requirements of traffic sign inventory management and environmental monitoring while falling short of catering to the demand for high-speed navigation. In particular, the Miller 2 encoding scheme strikes the best balance between reading performance (e.g., throughput) and noise tolerance for the multipath effect. Additionally, we show that the on-vehicle antenna should be oriented to maximize the available time for reading on-road tags, although it may reduce the received power by the tags in the forward link.