Future wireless networks are poised to transform into integrated sensing and communication (ISAC) networks, unlocking groundbreaking services such as digital twinning. To harness the full potential of ISAC networks, it is essential to experimentally validate their sensing capabilities and the role of sensing in boosting communication. However, current prototype systems fall short in supporting multiple sensing functions or validating sensing-assisted communication. In response, we have developed an advanced ISAC prototype system that incorporates monostatic, bistatic, and network sensing modes. This system supports multimodal data collection and synchronization, ensuring comprehensive experimental validation. On the communication front, it excels in sensing-aided beam tracking and real-time high-definition video transmission. For sensing applications, it provides precise angle and range measurements, real-time angle-range imaging, and radio-based simultaneous localization and mapping (SLAM). Our prototype aligns with the 5G New Radio standard, offering scalability for up to 16 user equipments (UEs) in uplink transmission and 10 UEs in downlink transmission. Real-world tests showcase the system's superior accuracy, with root mean square errors of 2.3 degrees for angle estimation and 0.3 meters (m) for range estimation. Additionally, the estimation errors for multimodal-aided real-time radio SLAM localization and mapping are 0.25 m and 0.8 m, respectively.