Knowledge Synthesis of Photosynthesis Research Using a Large Language Model

The development of biological data analysis tools and large language models (LLMs) has opened up new possibilities for utilizing AI in plant science research, with the potential to contribute significantly to knowledge integration and research gap identification. Nonetheless, current LLMs struggle to handle complex biological data and theoretical models in photosynthesis research and often fail to provide accurate scientific contexts. Therefore, this study proposed a photosynthesis research assistant (PRAG) based on OpenAI's GPT-4o with retrieval-augmented generation (RAG) techniques and prompt optimization. Vector databases and an automated feedback loop were used in the prompt optimization process to enhance the accuracy and relevance of the responses to photosynthesis-related queries. PRAG showed an average improvement of 8.7% across five metrics related to scientific writing, with a 25.4% increase in source transparency. Additionally, its scientific depth and domain coverage were comparable to those of photosynthesis research papers. A knowledge graph was used to structure PRAG's responses with papers within and outside the database, which allowed PRAG to match key entities with 63% and 39.5% of the database and test papers, respectively. PRAG can be applied for photosynthesis research and broader plant science domains, paving the way for more in-depth data analysis and predictive capabilities.

Melon Fruit Detection and Quality Assessment Using Generative AI-Based Image Data Augmentation

Monitoring and managing the growth and quality of fruits are very important tasks. To effectively train deep learning models like YOLO for real-time fruit detection, high-quality image datasets are essential. However, such datasets are often lacking in agriculture. Generative AI models can help create high-quality images. In this study, we used MidJourney and Firefly tools to generate images of melon greenhouses and post-harvest fruits through text-to-image, pre-harvest image-to-image, and post-harvest image-to-image methods. We evaluated these AIgenerated images using PSNR and SSIM metrics and tested the detection performance of the YOLOv9 model. We also assessed the net quality of real and generated fruits. Our results showed that generative AI could produce images very similar to real ones, especially for post-harvest fruits. The YOLOv9 model detected the generated images well, and the net quality was also measurable. This shows that generative AI can create realistic images useful for fruit detection and quality assessment, indicating its great potential in agriculture. This study highlights the potential of AI-generated images for data augmentation in melon fruit detection and quality assessment and envisions a positive future for generative AI applications in agriculture.