"So There's a Catch-22 Here": How Early Adopters Who Build Multi-Agent LLM Systems Conceptualize Transparency

Multi-agent large language model (LLM) systems are rapidly emerging, yet transparency, a cornerstone of responsible AI, remains under-defined in these distributed architectures, which have complexities of inter-agent coordination and orchestration. In this paper, we present one of the first empirical study of how early adopters of multi-agent LLM systems, who are both the builders and users, understand and practice transparency. We conducted semi-structured interviews with 13 early adopters in [Large Technology Organization] and applied thematic analysis to identify recurring patterns. Participants articulated divergent yet complementary framings of transparency, including reproducibility, debugging, boundary-setting, visualization, and auditing. These perspectives spanned questions of what transparency entails, why it matters, and how it is achieved. We synthesize these into a multidimensional framework, which is developer, user, and governance-focused positioning transparency as a situated socio-technical practice that informs future HCI and AI design and research around aligning expectations and capacities of their intended audiences.

BioAgents: Democratizing Bioinformatics Analysis with Multi-Agent Systems

Creating end-to-end bioinformatics workflows requires diverse domain expertise, which poses challenges for both junior and senior researchers as it demands a deep understanding of both genomics concepts and computational techniques. While large language models (LLMs) provide some assistance, they often fall short in providing the nuanced guidance needed to execute complex bioinformatics tasks, and require expensive computing resources to achieve high performance. We thus propose a multi-agent system built on small language models, fine-tuned on bioinformatics data, and enhanced with retrieval augmented generation (RAG). Our system, BioAgents, enables local operation and personalization using proprietary data. We observe performance comparable to human experts on conceptual genomics tasks, and suggest next steps to enhance code generation capabilities.

AI-Enhanced Sensemaking: Exploring the Design of a Generative AI-Based Assistant to Support Genetic Professionals

Generative AI has the potential to transform knowledge work, but further research is needed to understand how knowledge workers envision using and interacting with generative AI. We investigate the development of generative AI tools to support domain experts in knowledge work, examining task delegation and the design of human-AI interactions. Our research focused on designing a generative AI assistant to aid genetic professionals in analyzing whole genome sequences (WGS) and other clinical data for rare disease diagnosis. Through interviews with 17 genetics professionals, we identified current challenges in WGS analysis. We then conducted co-design sessions with six genetics professionals to determine tasks that could be supported by an AI assistant and considerations for designing interactions with the AI assistant. From our findings, we identified sensemaking as both a current challenge in WGS analysis and a process that could be supported by AI. We contribute an understanding of how domain experts envision interacting with generative AI in their knowledge work, a detailed empirical study of WGS analysis, and three design considerations for using generative AI to support domain experts in sensemaking during knowledge work. CCS CONCEPTS: Human-centered computing, Human-computer interaction, Empirical studies in HCI Additional Keywords and Phrases: whole genome sequencing, generative AI, large language models, knowledge work, sensemaking, co-design, rare disease Contact Author: Angela Mastrianni (This work was done during the author's internship at Microsoft Research) Ashley Mae Conard and Amanda K. Hall contributed equally