Lymphoid Infiltration Assessment of the Tumor Margins in H&E Slides

Lymphoid infiltration at tumor margins is a key prognostic marker in solid tumors, playing a crucial role in guiding immunotherapy decisions. Current assessment methods, heavily reliant on immunohistochemistry (IHC), face challenges in tumor margin delineation and are affected by tissue preservation conditions. In contrast, we propose a Hematoxylin and Eosin (H&E) staining-based approach, underpinned by an advanced lymphocyte segmentation model trained on a public dataset for the precise detection of CD3+ and CD20+ lymphocytes. In our colorectal cancer study, we demonstrate that our H&E-based method offers a compelling alternative to traditional IHC, achieving comparable results in many cases. Our method's validity is further explored through a Turing test, involving blinded assessments by a pathologist of anonymized curves from H&E and IHC slides. This approach invites the medical community to consider Turing tests as a standard for evaluating medical applications involving expert human evaluation, thereby opening new avenues for enhancing cancer management and immunotherapy planning.

Optimizing Lymphocyte Detection in Breast Cancer Whole Slide Imaging through Data-Centric Strategies

Efficient and precise quantification of lymphocytes in histopathology slides is imperative for the characterization of the tumor microenvironment and immunotherapy response insights. We developed a data-centric optimization pipeline that attain great lymphocyte detection performance using an off-the-shelf YOLOv5 model, without any architectural modifications. Our contribution that rely on strategic dataset augmentation strategies, includes novel biological upsampling and custom visual cohesion transformations tailored to the unique properties of tissue imagery, and enables to dramatically improve model performances. Our optimization reveals a pivotal realization: given intensive customization, standard computational pathology models can achieve high-capability biomarker development, without increasing the architectural complexity. We showcase the interest of this approach in the context of breast cancer where our strategies lead to good lymphocyte detection performances, echoing a broadly impactful paradigm shift. Furthermore, our data curation techniques enable crucial histological analysis benchmarks, highlighting improved generalizable potential.

Learning an Adaptation Function to Assess Image Visual Similarities

Human perception is routinely assessing the similarity between images, both for decision making and creative thinking. But the underlying cognitive process is not really well understood yet, hence difficult to be mimicked by computer vision systems. State-of-the-art approaches using deep architectures are often based on the comparison of images described as feature vectors learned for image categorization task. As a consequence, such features are powerful to compare semantically related images but not really efficient to compare images visually similar but semantically unrelated. Inspired by previous works on neural features adaptation to psycho-cognitive representations, we focus here on the specific task of learning visual image similarities when analogy matters. We propose to compare different supervised, semi-supervised and self-supervised networks, pre-trained on distinct scales and contents datasets (such as ImageNet-21k, ImageNet-1K or VGGFace2) to conclude which model may be the best to approximate the visual cortex and learn only an adaptation function corresponding to the approximation of the the primate IT cortex through the metric learning framework. Our experiments conducted on the Totally Looks Like image dataset highlight the interest of our method, by increasing the retrieval scores of the best model @1 by 2.25x. This research work was recently accepted for publication at the ICIP 2021 international conference [1]. In this new article, we expand on this previous work by using and comparing new pre-trained feature extractors on other datasets.