Reasonable setting of traffic signals can be very helpful in alleviating congestion in urban traffic networks. Meta-heuristic optimization algorithms have proved themselves to be able to find high-quality signal timing plans. However, they generally suffer from performance deterioration when solving large-scale traffic signal optimization problems due to the huge search space and limited computational budget. Directing against this issue, this study proposes a surrogate-assisted cooperative signal optimization (SCSO) method. Different from existing methods that directly deal with the entire traffic network, SCSO first decomposes it into a set of tractable sub-networks, and then achieves signal setting by cooperatively optimizing these sub-networks with a surrogate-assisted optimizer. The decomposition operation significantly narrows the search space of the whole traffic network, and the surrogate-assisted optimizer greatly lowers the computational burden by reducing the number of expensive traffic simulations. By taking Newman fast algorithm, radial basis function and a modified estimation of distribution algorithm as decomposer, surrogate model and optimizer, respectively, this study develops a concrete SCSO algorithm. To evaluate its effectiveness and efficiency, a large-scale traffic network involving crossroads and T-junctions is generated based on a real traffic network. Comparison with several existing meta-heuristic algorithms specially designed for traffic signal optimization demonstrates the superiority of SCSO in reducing the average delay time of vehicles.