Chinese Large Language Models (LLMs) have recently demonstrated impressive capabilities across various NLP benchmarks and real-world applications. However, the existing benchmarks for comprehensively evaluating these LLMs are still insufficient, particularly in terms of measuring knowledge that LLMs capture. Current datasets collect questions from Chinese examinations across different subjects and educational levels to address this issue. Yet, these benchmarks primarily focus on objective questions such as multiple-choice questions, leading to a lack of diversity in question types. To tackle this problem, we propose LHMKE, a Large-scale, Holistic, and Multi-subject Knowledge Evaluation benchmark in this paper. LHMKE is designed to provide a comprehensive evaluation of the knowledge acquisition capabilities of Chinese LLMs. It encompasses 10,465 questions across 75 tasks covering 30 subjects, ranging from primary school to professional certification exams. Notably, LHMKE includes both objective and subjective questions, offering a more holistic evaluation of the knowledge level of LLMs. We have assessed 11 Chinese LLMs under the zero-shot setting, which aligns with real examinations, and compared their performance across different subjects. We also conduct an in-depth analysis to check whether GPT-4 can automatically score subjective predictions. Our findings suggest that LHMKE is a challenging and advanced testbed for Chinese LLMs.