One primary topic of multi-modal learning is to jointly incorporate heterogeneous information from different modalities. However, most models often suffer from unsatisfactory multi-modal cooperation, which could not jointly utilize all modalities well. Some methods are proposed to identify and enhance the worse learnt modality, but are often hard to provide the fine-grained observation of multi-modal cooperation at sample-level with theoretical support. Hence, it is essential to reasonably observe and improve the fine-grained cooperation between modalities, especially when facing realistic scenarios where the modality discrepancy could vary across different samples. To this end, we introduce a fine-grained modality valuation metric to evaluate the contribution of each modality at sample-level. Via modality valuation, we regretfully observe that the multi-modal model tends to rely on one specific modality, resulting in other modalities being low-contributing. We further analyze this issue and improve cooperation between modalities by enhancing the discriminative ability of low-contributing modalities in a targeted manner. Overall, our methods reasonably observe the fine-grained uni-modal contribution at sample-level and achieve considerable improvement on different multi-modal models.