We present DocPuzzle, a rigorously constructed benchmark for evaluating long-context reasoning capabilities in large language models (LLMs). This benchmark comprises 100 expert-level QA problems requiring multi-step reasoning over long real-world documents. To ensure the task quality and complexity, we implement a human-AI collaborative annotation-validation pipeline. DocPuzzle introduces an innovative evaluation framework that mitigates guessing bias through checklist-guided process analysis, establishing new standards for assessing reasoning capacities in LLMs. Our evaluation results show that: 1)Advanced slow-thinking reasoning models like o1-preview(69.7%) and DeepSeek-R1(66.3%) significantly outperform best general instruct models like Claude 3.5 Sonnet(57.7%); 2)Distilled reasoning models like DeepSeek-R1-Distill-Qwen-32B(41.3%) falls far behind the teacher model, suggesting challenges to maintain the generalization of reasoning capabilities relying solely on distillation.