In personalized Federated Learning (pFL), high data heterogeneity can cause significant gradient divergence across devices, adversely affecting the learning process. This divergence, especially when gradients from different users form an obtuse angle during aggregation, can negate progress, leading to severe weight and gradient update degradation. To address this issue, we introduce a new approach to pFL design, namely Federated Learning with Layer-wise Aggregation via Gradient Analysis (FedLAG), utilizing the concept of gradient conflict at the layer level. Specifically, when layer-wise gradients of different clients form acute angles, those gradients align in the same direction, enabling updates across different clients toward identifying client-invariant features. Conversely, when layer-wise gradient pairs make create obtuse angles, the layers tend to focus on client-specific tasks. In hindsights, FedLAG assigns layers for personalization based on the extent of layer-wise gradient conflicts. Specifically, layers with gradient conflicts are excluded from the global aggregation process. The theoretical evaluation demonstrates that when integrated into other pFL baselines, FedLAG enhances pFL performance by a certain margin. Therefore, our proposed method achieves superior convergence behavior compared with other baselines. Extensive experiments show that our FedLAG outperforms several state-of-the-art methods and can be easily incorporated with many existing methods to further enhance performance.