Serialized output training (SOT) attracts increasing attention due to its convenience and flexibility for multi-speaker automatic speech recognition (ASR). However, it is not easy to train with attention loss only. In this paper, we propose the overlapped encoding separation (EncSep) to fully utilize the benefits of the connectionist temporal classification (CTC) and attention hybrid loss. This additional separator is inserted after the encoder to extract the multi-speaker information with CTC losses. Furthermore, we propose the serialized speech information guidance SOT (GEncSep) to further utilize the separated encodings. The separated streams are concatenated to provide single-speaker information to guide attention during decoding. The experimental results on LibriMix show that the single-speaker encoding can be separated from the overlapped encoding. The CTC loss helps to improve the encoder representation under complex scenarios. GEncSep further improved performance.