In this study, we present Q-Seg, a novel unsupervised image segmentation method based on quantum annealing, tailored for existing quantum hardware. We formulate the pixel-wise segmentation problem, which assimilates spectral and spatial information of the image, as a graph-cut optimization task. Our method efficiently leverages the interconnected qubit topology of the D-Wave Advantage device, offering superior scalability over existing quantum approaches and outperforming state-of-the-art classical methods. Our empirical evaluations on synthetic datasets reveal that Q-Seg offers better runtime performance against the classical optimizer Gurobi. Furthermore, we evaluate our method on segmentation of Earth Observation images, an area of application where the amount of labeled data is usually very limited. In this case, Q-Seg demonstrates near-optimal results in flood mapping detection with respect to classical supervised state-of-the-art machine learning methods. Also, Q-Seg provides enhanced segmentation for forest coverage compared to existing annotated masks. Thus, Q-Seg emerges as a viable alternative for real-world applications using available quantum hardware, particularly in scenarios where the lack of labeled data and computational runtime are critical.