This paper addresses the robust speech recognition problem as an adaptation task. Specifically, we investigate the cumulative application of adaptation methods. A bidirectional Long Short-Term Memory (BLSTM) based neural network, capable of learning temporal relationships and translation invariant representations, is used for robust acoustic modelling. Further, i-vectors were used as an input to the neural network to perform instantaneous speaker and environment adaptation, providing 8\% relative improvement in word error rate on the NIST Hub5 2000 evaluation test set. By enhancing the first-pass i-vector based adaptation with a second-pass adaptation using speaker and environment dependent transformations within the network, a further relative improvement of 5\% in word error rate was achieved. We have reevaluated the features used to estimate i-vectors and their normalization to achieve the best performance in a modern large scale automatic speech recognition system.