Artificial Intelligence(AI) widely applies in Image Classification and Recognition, Text Understanding and Natural Language Processing, which makes great progress. In this paper, we introduced AI into the fruit quality detection field. We designed a fruit sugar degree regression model using an Artificial Neural Network based on spectra of fruits within the visible/near-infrared(V/NIR)range. After analysis of fruit spectra, we innovatively proposed a new neural network structure: low layers consist of a Multilayer Perceptron(MLP), a middle layer is a 2-dimensional correlation matrix layer, and high layers consist of several Convolutional Neural Network(CNN) layers. In this study, we used fruit sugar value as a detection target, collecting two fruits called Gan Nan Navel and Tian Shan Pear as samples, doing experiments respectively, and comparing their results. We used Analysis of Variance(ANOVA) to evaluate the reliability of the dataset we collected. Then, we tried multiple strategies to process spectrum data, evaluating their effects. In this paper, we tried to add Wavelet Decomposition(WD) to reduce feature dimensions and a Genetic Algorithm(GA) to find excellent features. Then, we compared Neural Network models with traditional Partial Least Squares(PLS) based models. We also compared the neural network structure we designed(MLP-CNN) with other traditional neural network structures. In this paper, we proposed a new evaluation standard derived from dataset standard deviation(STD) for evaluating detection performance, validating the viability of using an artificial neural network model to do fruit sugar degree nondestructive detection.