Multi-modal object detection in autonomous driving has achieved great breakthroughs due to the usage of fusing complementary information from different sensors. The calibration in fusion between sensors such as LiDAR and camera is always supposed to be precise in previous work. However, in reality, calibration matrices are fixed when the vehicles leave the factory, but vibration, bumps, and data lags may cause calibration bias. As the research on the calibration influence on fusion detection performance is relatively few, flexible calibration dependency multi-sensor detection method has always been attractive. In this paper, we conducted experiments on SOTA detection method EPNet++ and proved slight bias on calibration can reduce the performance seriously. We also proposed a re-calibration model based on semantic segmentation which can be combined with a detection algorithm to improve the performance and robustness of multi-modal calibration bias.