Injectivity is the defining property of a mapping that ensures no information is lost and any input can be perfectly reconstructed from its output. By performing hard thresholding, the ReLU function naturally interferes with this property, making the injectivity analysis of ReLU-layers in neural networks a challenging yet intriguing task that has not yet been fully solved. This article establishes a frame theoretic perspective to approach this problem. The main objective is to develop the most general characterization of the injectivity behavior of ReLU-layers in terms of all three involved ingredients: (i) the weights, (ii) the bias, and (iii) the domain where the data is drawn from. Maintaining a focus on practical applications, we limit our attention to bounded domains and present two methods for numerically approximating a maximal bias for given weights and data domains. These methods provide sufficient conditions for the injectivity of a ReLU-layer on those domains and yield a novel practical methodology for studying the information loss in ReLU layers. Finally, we derive explicit reconstruction formulas based on the duality concept from frame theory.