Face alignment is a crucial step in preparing face images for feature extraction in facial analysis tasks. For applications such as face recognition, facial expression recognition, and facial attribute classification, alignment is widely utilized during both training and inference to standardize the positions of key landmarks in the face. It is well known that the application and method of face alignment significantly affect the performance of facial analysis models. However, the impact of alignment on face image quality has not been thoroughly investigated. Current FIQA studies often assume alignment as a prerequisite but do not explicitly evaluate how alignment affects quality metrics, especially with the advent of modern deep learning-based detectors that integrate detection and landmark localization. To address this need, our study examines the impact of face alignment on face image quality scores. We conducted experiments on the LFW, IJB-B, and SCFace datasets, employing MTCNN and RetinaFace models for face detection and alignment. To evaluate face image quality, we utilized several assessment methods, including SER-FIQ, FaceQAN, DifFIQA, and SDD-FIQA. Our analysis included examining quality score distributions for the LFW and IJB-B datasets and analyzing average quality scores at varying distances in the SCFace dataset. Our findings reveal that face image quality assessment methods are sensitive to alignment. Moreover, this sensitivity increases under challenging real-life conditions, highlighting the importance of evaluating alignment's role in quality assessment.