We investigate the decidability and computational complexity of conservative extensions and the related notions of inseparability and entailment in Horn description logics (DLs) with inverse roles. We consider both query conservative extensions, defined by requiring that the answers to all conjunctive queries are left unchanged, and deductive conservative extensions, which require that the entailed concept inclusions, role inclusions, and functionality assertions do not change. Upper bounds for query conservative extensions are particularly challenging because characterizations in terms of unbounded homomorphisms between universal models, which are the foundation of the standard approach to establishing decidability, fail in the presence of inverse roles. We resort to a characterization that carefully mixes unbounded and bounded homomorphisms and enables a decision procedure that combines tree automata and a mosaic technique. Our main results are that query conservative extensions are 2ExpTime-complete in all DLs between ELI and Horn-ALCHIF and between Horn-ALC and Horn-ALCHIF, and that deductive conservative extensions are 2ExpTime-complete in all DLs between ELI and ELHIF_\bot. The same results hold for inseparability and entailment.