Abstract:Hyperspectral (HS) imaging was successfully employed in the 380 nm to 1000 nm wavelength range to investigate the efficacy of detecting age, healthiness and individual nutrient deficiency of grapevine leaves collected from vineyards located in central west NSW, Australia. For age detection, the appearance of many healthy grapevine leaves has been examined. Then visually defective leaves were compared with healthy leaves. Control leaves and individual nutrient-deficient leaves (e.g. N, K and Mg) were also analysed. Several features were employed at various stages in the Ultraviolet (UV), Visible (VIS) and Near Infrared (NIR) regions to evaluate the experimental data: mean brightness, mean 1st derivative brightness, variation index, mean spectral ratio, normalised difference vegetation index (NDVI) and standard deviation (SD). Experiment results demonstrate that these features could be utilised with a high degree of effectiveness to compare age, identify unhealthy samples and not only to distinguish from control and nutrient deficiency but also to identify individual nutrient defects. Therefore, our work corroborated that HS imaging has excellent potential as a non-destructive as well as a non-contact method to detect age, healthiness and individual nutrient deficiencies of grapevine leaves