Development and validation of deep learning based embryo selection across multiple days of transfer

This work describes the development and validation of a fully automated deep learning model, iDAScore v2.0, for the evaluation of embryos incubated for 2, 3, and 5 or more days. The model is trained and evaluated on an extensive and diverse dataset including 181,428 embryos from 22 IVF clinics across the world. For discriminating transferred embryos with known outcome (KID), we show AUCs ranging from 0.621 to 0.708 depending on the day of transfer. Predictive performance increased over time and showed a strong correlation with morphokinetic parameters. The model has equivalent performance to KIDScore D3 on day 3 embryos while significantly surpassing the performance of KIDScore D5 v3 on day 5+ embryos. This model provides an analysis of time-lapse sequences without the need for user input, and provides a reliable method for ranking embryos for likelihood to implant, at both cleavage and blastocyst stages. This greatly improves embryo grading consistency and saves time compared to traditional embryo evaluation methods.

Robust and generalizable embryo selection based on artificial intelligence and time-lapse image sequences

Assessing and selecting the most viable embryos for transfer is an essential part of in vitro fertilization (IVF). In recent years, several approaches have been made to improve and automate the procedure using artificial intelligence (AI) and deep learning. Based on images of embryos with known implantation data (KID), AI models have been trained to automatically score embryos related to their chance of achieving a successful implantation. However, as of now, only limited research has been conducted to evaluate how embryo selection models generalize to new clinics and how they perform in subgroup analyses across various conditions. In this paper, we investigate how a deep learning-based embryo selection model using only time-lapse image sequences performs across different patient ages and clinical conditions, and how it correlates with traditional morphokinetic parameters. The model was trained and evaluated based on a large dataset from 18 IVF centers consisting of 115,832 embryos, of which 14,644 embryos were transferred KID embryos. In an independent test set, the AI model sorted KID embryos with an area under the curve (AUC) of a receiver operating characteristic curve of 0.67 and all embryos with an AUC of 0.95. A clinic hold-out test showed that the model generalized to new clinics with an AUC range of 0.60-0.75 for KID embryos. Across different subgroups of age, insemination method, incubation time, and transfer protocol, the AUC ranged between 0.63 and 0.69. Furthermore, model predictions correlated positively with blastocyst grading and negatively with direct cleavages. The fully automated iDAScore v1.0 model was shown to perform at least as good as a state-of-the-art manual embryo selection model. Moreover, full automatization of embryo scoring implies fewer manual evaluations and eliminates biases due to inter- and intraobserver variation.