We present a complete methodology for authenticating local bee pollen against fraudulent samples using image processing and machine learning techniques. The proposed standard methods do not need expensive equipment such as advanced microscopes and can be used for a preliminary fast rejection of unknown pollen types. The system is able to rapidly reject the non-local pollen samples with inexpensive hardware and without the need to send the product to the laboratory. Methods are based on the color properties of bee pollen loads images and the use of one-class classifiers which are appropriate to reject unknown pollen samples when there is limited data about them. The validation of the method is carried out by authenticating Spanish bee pollen types. Experimentation shows that the proposed methods can obtain an overall authentication accuracy of 94%. We finally illustrate the user interaction with the software in some practical cases by showing the developed application prototype.