This paper studies semantic-aware communication for remote estimation of multiple Markov sources over a lossy and rate-constrained channel. Unlike most existing studies that treat all source states equally, we exploit the semantics of information and consider that the remote actuator has different tolerances for the estimation errors of different states. We aim to find an optimal scheduling policy that minimizes the long-term state-dependent costs of estimation errors under a transmission frequency constraint. We theoretically show the structure of the optimal policy by leveraging the average-cost Constrained Markov Decision Process (CMDP) theory and the Lagrangian dynamic programming. By exploiting the optimal structural results, we develop a novel policy search algorithm, termed intersection search plus relative value iteration (Insec-RVI), that can find the optimal policy using only a few iterations. To avoid the ``curse of dimensionality'' of MDPs, we propose an online low-complexity drift-plus-penalty (DPP) scheduling algorithm based on the Lyapunov optimization theorem. We also design an efficient average-cost Q-learning algorithm to estimate the optimal policy without knowing a priori the channel and source statistics. Numerical results show that continuous transmission is inefficient, and remarkably, our semantic-aware policies can attain the optimum by strategically utilizing fewer transmissions by exploiting the timing of the important information.