This volume consists of papers presented at the Sixteenth Conference on Theoretical Aspects of Rationality and Knowledge (TARK) held at the University of Liverpool, UK, from July 24 to 26, 2017. TARK conferences bring together researchers from a wide variety of fields, including Computer Science (especially, Artificial Intelligence, Cryptography, Distributed Computing), Economics (especially, Decision Theory, Game Theory, Social Choice Theory), Linguistics, Philosophy (especially, Philosophical Logic), and Cognitive Psychology, in order to further understand the issues involving reasoning about rationality and knowledge.