The internet of things (IoT) based wireless sensor networks (WSNs) face an energy shortage challenge that could be overcome by the novel wireless power transfer (WPT) technology. The combination of WSNs and WPT is known as wireless rechargeable sensor networks (WRSNs), with the charging efficiency and charging scheduling being the primary concerns. Therefore, this paper proposes a probabilistic on-demand charging scheduling for integrated sensing and communication (ISAC)-assisted WRSNs with multiple mobile charging vehicles (MCVs) that addresses three parts. First, it considers the four attributes with their probability distributions to balance the charging load on each MCV. The distributions are residual energy of charging node, distance from MCV to charging node, degree of charging node, and charging node betweenness centrality. Second, it considers the efficient charging factor strategy to partially charge network nodes. Finally, it employs the ISAC concept to efficiently utilize the wireless resources to reduce the traveling cost of each MCV and to avoid the charging conflicts between them. The simulation results show that the proposed protocol outperforms cutting-edge protocols in terms of energy usage efficiency, charging delay, survival rate, and travel distance.