In this paper, an interference cancellation based neural receiver for superimposed pilot (SIP) in multi-layer transmission is proposed, where the data and pilot are non-orthogonally superimposed in the same time-frequency resource. Specifically, to deal with the intra-layer and inter-layer interference of SIP under multi-layer transmission, the interference cancellation with superimposed symbol aided channel estimation is leveraged in the neural receiver, accompanied by the pre-design of pilot code-division orthogonal mechanism at transmitter. In addition, to address the complexity issue for inter-vendor collaboration and the generalization problem in practical deployments, respectively, this paper also provides a fixed SIP (F-SIP) design based on constant pilot power ratio and scalable mechanisms for different modulation and coding schemes (MCSs) and transmission layers. Simulation results demonstrate the superiority of the proposed schemes on the performance of block error rate and throughput compared with existing counterparts.