In recent years, the remarkable advancements in deep neural networks have brought tremendous convenience. However, the training process of a highly effective model necessitates a substantial quantity of samples, which brings huge potential threats, like unauthorized exploitation with privacy leakage. In response, we propose a framework named HiddenSpeaker, embedding imperceptible perturbations within the training speech samples and rendering them unlearnable for deep-learning-based speaker verification systems that employ large-scale speakers for efficient training. The HiddenSpeaker utilizes a simplified error-minimizing method named Single-Level Error-Minimizing (SLEM) to generate specific and effective perturbations. Additionally, a hybrid objective function is employed for human perceptual optimization, ensuring the perturbation is indistinguishable from human listeners. We conduct extensive experiments on multiple state-of-the-art (SOTA) models in the speaker verification domain to evaluate HiddenSpeaker. Our results demonstrate that HiddenSpeaker not only deceives the model with unlearnable samples but also enhances the imperceptibility of the perturbations, showcasing strong transferability across different models.