This paper provides a new unimodality test with application in hierarchical clustering methods. The proposed method denoted by signature test (Sigtest), transforms the data based on its statistics. The transformed data has much smaller variation compared to the original data and can be evaluated in a simple proposed unimodality test. Compared with the existing unimodality tests, Sigtest is more accurate in detecting the overlapped clusters and has a much less computational complexity. Simulation results demonstrate the efficiency of this statistic test for both real and synthetic data sets.