Using agar plates hosting a 2D cell population stimulated with signaling molecules is crucial for experiments such as gene regulation and drug discovery in a wide range of biological studies. In this paper, a biophysical model is proposed that incorporates droplet soaking, diffusion of molecules within agar, cell growth over an agar surface, and absorption of signaling molecules by cells. The proposed model describes the channel response and provides valuable insights for designing experiments more efficiently and accurately. The molecule release rate due to droplet soaking into agar, which is characterized and modeled as the source term for the diffusion model, is derived. Furthermore, cell growth is considered over the surface, which dictates the dynamics of signaling molecule reactions and leads to a variable boundary condition. As a case study, genetically-modified $E.\,coli$ bacteria are spread over the surface of agar and Isopropyl-beta-D thiogalactopyranoside (IPTG) is considered as a signaling molecule. IPTG droplets are dropped onto the bacteria-covered agar surface. The parameters for the IPTG molecule release rate as a diffusion source into the agar are estimated from this experiment. Then, a particle-based simulator is used to obtain the spatio-temporal profile of the signaling molecules received by the surface bacteria. The results indicate that the number of molecules reacting with or absorbed by bacteria at different locations on the surface could be widely different, which highlights the importance of taking this variation into account for biological inferences.