There is a growing interest in shape analysis in recent years and in this paper we present a novel contour-based shape representation named Beltrami signature for 2D bounded simple connected domain. The proposed representation is based on conformal welding. With suitable normalization, the uniqueness of welding is guaranteed up to a rotation. Then it can be extended to a harmonic function and finally quasi-conformal theory get rid of the only uncertainty by computing Beltrami coefficient of harmonic extension. The benifits of the proposed signature is it keeps invariant under simple transformations like sacling, transformation and rotation and is roubost under slight deformation and distortion. Experiments demonstrates the above properties and also shows the excellent classification performance.