Background: Implementing a standardized 31P-MRS dynamic acquisition protocol to evaluate skeletal muscle energy metabolism and monitor muscle fatigability1,2, while being compatible with various longitudinal clinical studies on diversified patient cohorts, requires a high level of technicality and expertise. Furthermore, processing data to obtain reliable results also demands a great degree of expertise from the operator. In this two-part article, we present an advanced quality control approach for data acquired using a dynamic 31P-MRS protocol. The aim is to provide decision support to the operator in order to assist in data processing and obtain reliable results based on objective criteria. We present first in part one, an advanced data quality control (QC) approach of a dynamic 31P-MRS protocol. Part two is an impact study demonstrating the added value of the QC approach to explore clinical results derived from two patient populations with significant fatigue: COVID19 and multiple sclerosis (MS). Experimental: 31P-MRS was performed on a 3T clinical MRI in 175 subjects from clinical and healthy control populations conducted in a University Hospital. An advanced data QC Score (QCS) was developed using multiple objective criteria. The criteria were based on current recommendations from the literature enriched by new proposals based on clinical experience. The QCS was designed to indicate valid and corrupt data and guide necessary objective data editing to extract as much valid physiological data as possible. Dynamic acquisitions using an MR-compatible ergometer ran over a rest(40s), exercise(2min), and a recovery phase(6min). Results: Using QCS enabled rapid identification of subjects with data anomalies allowing the user to correct the data series or reject them partially or entirely as well as identify fully valid datasets. Overall, the use of the QCS resulted in the automatic classification of 45% of the subjects including 58 participants that had data with no criterion violation and 21 participants with violations that resulted in the rejection of all dynamic data. The remaining datasets were inspected manually with guidance allowing acceptance of full datasets from an additional 80 participants and recovery phase data from an additional 16 subjects. Overall, more anomalies occurred with patient data (35% of datasets) compared to healthy controls (15% of datasets). Conclusion: This paper describes typical difficulties encountered during the dynamic acquisition of 31P-MRS. Based on these observations, a standardized data quality control pipeline was created and implemented in both healthy and patient populations. The QC scoring ensures a standardized data rejection procedure and rigorous objective analysis of dynamic 31P-MRS data obtained from patients. The contribution of this methodology contributes to efforts made to standardize the practices of the 31P-MRS that has been underway for a decade, with the ultimate goal of making it an empowered tool for clinical research.